ࡱ> *,)q :bjbjt+t+  AAX]\\\\\\\   6888=u  $  Q\ @\\@@@*\\6p\\\\6@@6\\6 hҾR6Productivity Experiments Lipsen, Vestfals and Quenneville (IOS and UBC): Line P Monitoring As a continuation of our work in 1998, the principal focus along line P was to measure 14C incorporation into organic and inorganic particulate carbon. Size fractionated chlorophyll a concentrations were also measured at the 6 depths of the productivity samples as well as biogenic silica. P v I curves were also generated from each major station at 55% light depth. Samples were collected at all major stations. Again as a continuation of last years cruise work, we took samples from all major P stations for epifluorescence microscopy, inverted microscopy and scanning electron microscopy in order to enumerate the principal producers of organic and inorganic particulate carbon. Phytoplankton net (30 mm mesh) samples were taken from line P stations at the 1% light level as well as 150 m. Samples were preserved immediately for qualitative analysis. Initial examination will focus on the larger phytoplankton species with an emphasis on diatoms. An experiment was conducted to examine the influence of iron and light limitation of phytoplankton at station P26. Water from station P26 was collected using a teflon pump, supplemented with iron and incubated at various light intensities in the deck mounted incubators, together with appropriate non-supplemented controls. These were incubated for over 1 week and samples taken for chlorophyll, species composition, primary productivity, nutrients and dissolved iron. Isotope Inventory 5 mCi of 14C bicarbonate were taken on board. The whole quantity was used in experiments. Low level liquid waste was diluted and disposed over the side following set protocols. No 14C will be returned to shore with the exception of low level solid waste and filter material. One mCi of 32Si was brought on board. 0.91 mCi was used in experiments. Liquid and low level solid waste will be dealt with as above. The remaining 0.09 mCi will be transported back to UBC packed in an appropriate container. A. Pea (IOS): Line P Field experiments were initiated as part of a study of processes regulating the variability of primary production and of carbon fluxes along Line P transect. The main focus was at Station P26 and P4; the two-end members of Line P transect, where experiments were conducted to examine the influence of light intensity on changes in phytoplankton carbon to chlorophyll ratios. Water was collected using 10L GOFLO bottles at two depths (55% and 3.5% light depth) and diluted with filtered sea water from same depth. One set of samples was inoculated with 14C. All samples were incubated at 6 light intensities in on-deck incubators. Also, chlorophyll and phytoplankton samples for microscopic analyses (epifluorescence and inverted microscope) were obtained from 6 depths for determination of species composition and carbon content. In all major P stations, samples were collected at 2 depths (55 and 3.5% light depth) for photosynthesis vs. irradiance measurements and chlorophyll concentration. Eddy site With M. Quenneville (IOS), primary production was measured at 6 depths at two stations, one outside and one at the center of an eddy in an attempt to document potential enhancement of primary production by eddies. Isotope Inventory 10 mCi of 14C bicarbonate was brought on board. 6 mCi were used in the above experiments and 3 mCi in size fractionated primary production work of UBC/ IOS. The remaining 1 mCi was transported back to IOS. Low level liquid waste was diluted and disposed over the side following set protocols. Only 14C in low level solid waste and filter material was returned to shore. . 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